P-21: Sodium Selenite Improves the In vitroFollicular Development by Reducing the ReactiveOxygen Species Level and Increasing theTotal Antioxidant Capacity and GlutathioPeroxide Activity

Background: The aim of this study was to investigate the compare of Conventional (CV) and Direct Cover (DCV) vitrification and effect of sodium selenite(SS) that supplemented with FBS and BSA on reactive oxygen species (ROS) production, total antioxidant capacity (TAC) and glutathione peroxide (GPx) activity of cultured pre-antral follicles derived from vitrified and non-vitrified ovarian tissue. Materials and Methods: Immature mouse ovaries were vitrified (convevtional and direct cover methods), and mechanically isolated pre-antral follicles from vitrified and non-vitrified samples were cultured in TCM 199 medium with FBS and BSA and supplemented with different concentrations (0, 5 and 10 ng/ml) of SS. Follicular, oocyte and embryo development was assessed. In parallel, ROS, TAC and GPx levels were analyzed after 0, 12, 24, 48, 72 and 96 h of culture. Results: The normality of primary and preantral follicles in CV groups were higher than those achieved by DCV groups(p